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Updated: Feb 24, 2026

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Homeodomain Protein Transforming Growth Factor Beta-Induced Factor 2 Like, X-Linked Function in Colon Adenocarcinoma

Abolfazl Akbari1, Shahram Agah, Mansour Heidari

  • 1Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran.

Asian Pacific Journal of Cancer Prevention : APJCP
|August 28, 2017
PubMed

Insights

Transforming growth factor beta-induced factor 2 like, X-linked (TGIF2LX) acts as a tumor suppressor by inhibiting colon adenocarcinoma cell proliferation and angiogenesis. This finding suggests TGIF2LX as a potential target for colon cancer gene therapy.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • TGIF2LX (transforming growth factor beta-induced factor 2 like, X-linked) is a homeodomain protein involved in negative regulation of cell signaling.
  • Colon adenocarcinoma is a significant health concern, necessitating research into novel therapeutic targets.

Purpose of the Study:

  • To investigate the functional role of TGIF2LX in colon adenocarcinoma cells.
  • To explore TGIF2LX as a potential therapeutic target for colon cancer.

Main Methods:

  • Overexpression of wild-type TGIF2LX in SW48 colon adenocarcinoma cells.
  • Assessment of cell proliferation using MTT and BrdU assays.
  • In vivo tumor xenograft model in nude mice with immunohistochemical analysis for Ki-67 and CD34.
  • In silico analysis to predict TGIF2LX protein interactions.

Main Results:

  • TGIF2LX overexpression significantly inhibited colon tumor cell proliferation and angiogenesis (P<0.05).
  • In silico analysis predicted interactions between TGIF2LX and proteins crucial for cellular development.
  • TGIF2LX demonstrated potential as a transcription factor influencing tumor growth.

Conclusions:

  • TGIF2LX functions as a tumor suppressor in colon adenocarcinoma.
  • The findings elucidate molecular mechanisms of TGIF2LX's action in colon cancer.
  • TGIF2LX represents a promising candidate for future colon cancer gene-based therapeutic strategies.
Keywords:
CD34Homeodomain protein TGIF2LXHOX geneKi-67Transforming growth factor-beta (TGF-β)

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