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Proteomic profiling of human HepG2 cells treated with hesperidin using antibody array
Silvia Yumnam1, Venu Venkatarame Gowda Saralamma1, Suchismita Raha1
1Research Institute of Life Science, College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongsang 52828, Republic of Korea.
Molecular Medicine Reports
|August 30, 2017
Summary
This study used antibody arrays to identify proteins affected by hesperidin in HepG2 cells, revealing key proteins involved in cell death pathways. These findings offer new insights into hesperidin's anticancer mechanisms.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Oncology
Background:
- Hesperidin is a flavonoid with potential anticancer properties.
- Understanding the molecular mechanisms of hesperidin-induced cell death is crucial for its therapeutic development.
- HepG2 cells are a widely used model for studying liver cancer and drug responses.
Purpose of the Study:
- To identify and characterize proteins involved in hesperidin-induced cell death in HepG2 cells using antibody array technology.
- To elucidate the anticancer mechanism of hesperidin by analyzing protein expression changes.
Main Methods:
- Antibody array analysis (RayBio® Label-based L-series human antibody array) was employed to compare protein expression profiles between hesperidin-treated and untreated HepG2 cells.
- Western blot analysis was used for validation of identified proteins.
- STRING software was utilized for protein-protein interaction analysis.
Main Results:
- A range of proteins associated with signal transduction, cellular mechanisms, and cell growth/proliferation regulation were identified.
- Significant reductions in Heat Shock Protein 90 (Hsp90), Smac/DIABLO, Peroxiredoxin 6 (Prdx6), and Fyn-related kinase (FRK) were observed in hesperidin-treated cells.
- Protein-protein interaction analysis provided insights into the network of affected proteins.
Conclusions:
- This study is the first to utilize antibody array technology to identify protein markers associated with hesperidin-induced cell death in HepG2 cells.
- The findings provide novel insights into the anticancer mechanisms of hesperidin, highlighting the roles of specific proteins in its cytotoxic effects.
- The identified proteins may serve as potential biomarkers for hesperidin's efficacy.
