Drug-Delivery System Based on Salmon DNA Nano- and Micro-Scale Structures

Area of Science:

• Biomaterials Science
• Drug Delivery Systems
• Nanotechnology

Background:

• Microneedles offer painless drug self-administration.
• Novel materials are needed for advanced drug delivery vehicles.
• Salmon DNA (SDNA) presents unique properties for biomedical applications.

Purpose of the Study:

• To develop and evaluate double-stranded salmon DNA (SDNA) microneedles for drug delivery.
• To assess SDNA's suitability as both a structural material and drug carrier.
• To investigate the stability and efficacy of SDNA microneedles for drug encapsulation and skin permeation.

Main Methods:

• Fabrication of SDNA microneedles using nano-moulding technology under benign conditions.
• Characterization of SDNA microneedle properties including mechanical robustness, mouldability, and biocompatibility.
• Encapsulation of doxorubicin hydrochloride and assessment of drug-molecule binding stability using UV-absorption and photoluminescence.
• In vitro evaluation of microneedle mechanical functionality and skin penetration using COMSOL simulation and porcine skin models.
• Assessment of microneedle dissolution and drug permeation using rhodamine as a surrogate.

Main Results:

• SDNA microneedles were successfully fabricated with a high aspect ratio (4:1) using benign conditions.
• SDNA demonstrated excellent biocompatibility, bio-absorbability, mechanical robustness, and mouldability.
• Stable encapsulation of doxorubicin hydrochloride within the SDNA matrix was confirmed.
• In vitro studies showed effective penetration of porcine stratum corneum and successful drug permeation upon microneedle dissolution.

Conclusions:

• SDNA microneedles represent a promising, patient-friendly platform for drug delivery.
• The biocompatible and biodegradable nature of SDNA enhances its potential for therapeutic applications.
• SDNA microneedles offer a viable alternative to conventional drug delivery methods.