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Updated: Feb 23, 2026
Nucleofection of Rodent Neuroblasts to Study Neuroblast Migration In vitro
Published on: November 12, 2013
Recombinant neuritin affects the senescence, apoptosis, proliferation, and migration of rat bone marrow-derived
Wumei Yuan1, Lijuan Cui2, Guoxiang Li1
1The Key Laboratory of Xinjiang Endemic & Ethnic Diseases, Department of Biochemistry, Shihezi University School of Medicine, Shihezi, 832002, Xinjiang, China.
Objective:
To study the effects of recombinant neuritin expressed by Pichia pastoris GS115 on the senescence, apoptosis, proliferation, and migration associated with rat bone marrow-derived mesenchymal stem cells (BMSCs).
Results:
Recombinant neuritin was purified by Ni-affinity chromatography and identified by western blot and MALDI-TOF spectrometry. The effects of recombinant neuritin on senescence, apoptosis, proliferation, and migration of rat BMSCs WERE investigated. β-Galactosidase staining indicated that recombinant neuritin administration significantly inhibited BMSCs senescence at 1 μg neuritin/ml. Additionally, recombinant neuritin reduced the number of apoptotic cells at the early stage according to Annexin V/propidium iodide staining and inhibited cell proliferation according to MTT assay results. Moreover wound healing assay results showed that recombinant neuritin promoted BMSCs migration in the neuritin-treatment group.
Conclusion:
Recombinant neuritin affects the senescence, apoptosis, proliferation, migration of rat BMSCs. Our findings offer insight into neuritin function outside of the nervous system.
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