Determination of carazolol in tissues of pigs by high-performance liquid chromatography

A new and sensitive method is described for the determination of the beta-receptor blocker Carazolol, 4-(2-hydroxy-3-isopropylaminopropoxy)-carbazole, in different animal tissues. The procedure comprises extraction of Carazolol from tissue, clean-up by Kieselgel adsorption and high-performance liquid chromatographic separation with fluorimetric detection. The determination limit is 0.48 microgram/kg sample. The method has been verified by measuring Carazolol in liver, kidney and filet of a fattening pig treated with Carazolol before slaughtering.