Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Network Analysis of Medication Literacy and Associated Psychological Factors in Patients with Coronary Heart Disease and Diabetes.

Patient preference and adherence·2026

Same author

Rhein reduces conjugation of IncFII-type plasmids in <i>Escherichia coli</i> and mitigates the spread of antibiotic resistance genes.

Microbiology spectrum·2026

Same author

Baicalein activates the type I interferon signaling pathway to inhibit infectious bronchitis virus infection.

Poultry science·2026

Same author

A Toolkit for Targeted Neuromodulation of Striatal Direct Pathway Neurons Rescues Parkinsonian Motor Deficits in Mice.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Work stressors, work-related quality of life, psychological capital, and innovative behavior in emergency and critical care nurses: a cross-sectional moderated mediation study.

Frontiers in psychology·2026

Same author

PK/PD study of vancomycin in enterococcal infections: efficacy and safety assessment under the guidance of TDM.

Microbiology spectrum·2026

Search research articles

Related Experiment Video

Updated: Feb 23, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Multiplexed quantification of proteins and transcripts in single cells.

Vanessa M Peterson¹, Kelvin Xi Zhang², Namit Kumar¹

¹Genetics &Pharmacogenomics, Department of Translational Medicine, Merck &Co., Inc., Boston, Massachusetts, USA.

Nature Biotechnology

|August 31, 2017

Summary

This summary is machine-generated.

We developed a new tool, RNA expression and protein sequencing assay (REAP-seq), to simultaneously measure gene and protein levels in single cells. This method allows for comprehensive cellular analysis in a single workflow.

More Related Videos

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Related Experiment Videos

Last Updated: Feb 23, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Area of Science:

Single-cell biology
Molecular biology
Immunology

Background:

Accurate measurement of gene and protein expression is crucial for understanding cellular function.
Existing methods often require separate workflows for gene and protein analysis, limiting throughput and comprehensive analysis.
Single-cell resolution is key to dissecting cellular heterogeneity and identifying rare cell populations.

Purpose of the Study:

To introduce a novel assay, RNA expression and protein sequencing assay (REAP-seq), for simultaneous quantification of gene and protein expression in single cells.
To demonstrate the utility of REAP-seq in a biological context, including assessing drug effects and discovering novel cell types.

Main Methods:

Development and application of REAP-seq, integrating DNA-labeled antibodies and droplet microfluidics.
Quantification of >20,000 genes and proteins using 82 barcoded antibodies in a single workflow.
Application of REAP-seq to analyze human CD8+ lymphocytes and investigate the effects of a CD27 agonist.

Main Results:

Successful simultaneous measurement of gene and protein expression at the single-cell level.
Characterization of the costimulatory effects of a CD27 agonist on human CD8+ lymphocytes.
Identification and characterization of a previously unknown cell type within the analyzed samples.

Conclusions:

REAP-seq provides a powerful, integrated approach for high-throughput single-cell multi-omics analysis.
This assay enables deeper insights into cellular responses and facilitates the discovery of novel cellular identities.
REAP-seq has broad applications in immunology, cancer research, and developmental biology.