Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microtubule Instability02:17

Microtubule Instability

6.3K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
6.3K
GTPases and their Regulation02:14

GTPases and their Regulation

10.0K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
10.0K
GTPases and their Regulation02:14

GTPases and their Regulation

3.1K
3.1K
Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

6.0K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
6.0K
Destabilization of Microtubules01:45

Destabilization of Microtubules

3.7K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
3.7K
Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

11.8K
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
11.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plant kinesins: a bottom-up approach - from single molecules to function.

Journal of experimental botany·2026
Same author

Partial Depletion of SART1 Selectively Induces Mitotic Defects and Cell Death in Cancer Cells.

Genes to cells : devoted to molecular & cellular mechanisms·2026
Same author

Divergent toxicity mechanisms of amyloid-beta aggregates arising from a single aggregation reaction.

Cell reports·2026
Same author

Pathologies at the gateway: exploring the link between nucleoporins and inherited diseases.

Cellular and molecular life sciences : CMLS·2026
Same author

I was set to lead an undergraduate research trip abroad. Then my visa was denied.

Nature·2026
Same author

Germline fate determination by a single ARGONAUTE protein in <i>Ectocarpus</i>.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Feb 23, 2026

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

11.3K

Developmentally Regulated GTP binding protein 1 (DRG1) controls microtubule dynamics.

Anna Katharina Schellhaus1,2, Daniel Moreno-Andrés1,2, Mayank Chugh3

  • 1Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, 72076, Tübingen, Germany.

Scientific Reports
|September 1, 2017
PubMed
Summary
This summary is machine-generated.

Developmentally regulated GTP binding protein 1 (DRG1) binds microtubules and aids their function in cell division. Reduced DRG1 levels delay cell cycle progression by slowing spindle formation.

More Related Videos

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
07:20

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Published on: February 18, 2022

3.1K
High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
10:23

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

Published on: April 20, 2017

10.0K

Related Experiment Videos

Last Updated: Feb 23, 2026

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

11.3K
Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
07:20

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Published on: February 18, 2022

3.1K
High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
10:23

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

Published on: April 20, 2017

10.0K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cytoskeleton Dynamics

Background:

  • The mitotic spindle, crucial for cell division, comprises dynamic microtubules.
  • Microtubule dynamics are modulated by microtubule-associated proteins.

Purpose of the Study:

  • To identify and characterize novel microtubule-associated proteins.
  • To investigate the role of Developmentally regulated GTP binding protein 1 (DRG1) in microtubule function and cell division.

Main Methods:

  • In vitro biochemical assays to assess DRG1's interaction with microtubules.
  • Cellular studies using HeLa cells with reduced DRG1 levels to observe effects on mitosis.
  • Analysis of DRG1's domains for functional contribution.

Main Results:

  • DRG1 is identified as a microtubule-binding protein.
  • In vitro, DRG1 promotes microtubule polymerization, bundling, and stabilization.
  • Depletion of DRG1 in HeLa cells leads to delayed mitotic progression due to impaired spindle formation.
  • DRG1's functions do not require GTP hydrolysis, but all domains are essential.

Conclusions:

  • DRG1 plays a significant role in regulating microtubule dynamics and mitotic spindle assembly.
  • DRG1 is a novel microtubule-associated protein essential for efficient cell division.