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Fluorescence depth estimation from wide-field optical imaging data for guiding brain tumor resection: a
Dennis Wirth1, Kolbein Kolste2, Stephen Kanick2
1Department of Surgery, Dartmouth-Hitchcock Medical Center, 1 Medical Center Dr. Lebanon, NH 03766, USA.
Biomedical Optics Express
|September 1, 2017
Summary
This study enhances red light imaging for better tumor visualization during surgery. The technique can now distinguish fluorescence signals at different depths, improving surgical guidance.
Area of Science:
- Biomedical optics
- Surgical imaging
Background:
- Fluorescent agents aid tumor demarcation and surgical guidance.
- Challenges exist in visualizing fluorescence below the surface or through blood.
Purpose of the Study:
- Evaluate red light imaging for estimating fluorescent depths in turbid media.
- Investigate resolving multiple fluorescent emissions at varying depths and concentrations.
Main Methods:
- Utilized a broadband white light source for reflectance imaging.
- Excited Alexa Fluor 647 fluorescence with a 635 nm diode laser.
- Acquired spectral data (670-720 nm) using a liquid crystal tunable filter and sCMOS camera.
Main Results:
- Successfully resolved two fluorescent emissions within 2 mm in the same plane.
- Achieved resolution of emissions within 3 mm at depths up to 6 mm.
Conclusions:
- Red light imaging techniques show promise for improved depth estimation of fluorescent tumors.
- Enhanced visualization capabilities can lead to more precise surgical resections.
