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Related Experiment Video

Updated: Feb 23, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
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Line scanning, stage scanning confocal microscope (LSSSCM).

Daniel S Gareau1, James G Krueger1, Jason E Hawkes1

  • 1Investigative Dermatology, The Rockefeller University, 1230 York Ave., New York, NY 10065, USA.

Biomedical Optics Express
|September 1, 2017
PubMed
Summary

A novel line scanning, stage scanning confocal microscope (LSSSCM) offers rapid pathological assessment of large tissue samples. This innovative microscopy technique achieves cellular resolution, even in dense tissues like basal cell carcinoma.

Keywords:
(110.0180) Microscopy(170.1790) Confocal microscopy(170.1870) Dermatology(170.5810) Scanning microscopy(170.7050) Turbid media

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Area of Science:

  • Biomedical Engineering
  • Optical Microscopy
  • Pathology

Background:

  • Pathological assessment of large surgical excisions requires high cellular resolution.
  • Existing microscopy techniques can be slow or lack the necessary resolution for rapid assessment.

Purpose of the Study:

  • To introduce a novel microscope for rapid pathological assessment of large tissue samples.
  • To demonstrate cellular resolution imaging without scanning mirrors.

Main Methods:

  • Development of a line scanning, stage scanning confocal microscope (LSSSCM).
  • Utilizes a single cylindrical lens to focus laser light into a line.
  • Employs a single mechanical drive for stage movement (X and Y axes).
  • Generates semi-confocal optical sections.

Main Results:

  • LSSSCM achieves diffraction-limited line focus without scanning mirrors.
  • Demonstrates cellular resolution in high nuclear density conditions.
  • Successfully imaged micronodular basal cell carcinoma with cellular detail.

Conclusions:

  • LSSSCM provides a new method for rapid, high-resolution pathological assessment.
  • The mirrorless design simplifies the optical path and potentially increases speed.
  • This technology has potential applications in surgical pathology and research.