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Updated: Feb 23, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
Published on: August 11, 2020
Line scanning, stage scanning confocal microscope (LSSSCM)
Daniel S Gareau1, James G Krueger1, Jason E Hawkes1
1Investigative Dermatology, The Rockefeller University, 1230 York Ave., New York, NY 10065, USA.
Abstract:
For rapid pathological assessment of large surgical tissue excisions with cellular resolution, we present a line scanning, stage scanning confocal microscope (LSSSCM). LSSSCM uses no scanning mirrors. Laser light is focused with a single cylindrical lens to a line of diffraction-limited width directly into the (Z) sample focal plane, which is parallel to and near the flattened specimen surface. Semi-confocal optical sections are derived from the linear array distribution (Y) and a single mechanical drive that moves the sample parallel to the focal plane and perpendicular to the focused line (X). LSSSCM demonstrates cellular resolution in the conditions of high nuclear density within micronodular basal cell carcinoma.
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