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Advances in Identification and Mapping of Rust Resistance Genes in Wheat
Urmil Bansal1, Harbans Bariana2
1The University of Sydney Plant Breeding Institute, 107 Cobbitty Road, Cobbitty, NSW, 2570, Australia. urmil.bansal@sydney.edu.au.
Abstract:
Genetic characterisation of new rust resistance loci in wheat using cytogenetic/low-throughput genotyping systems required at least 5 years. Development of next-generation sequencing (NGS) based molecular marker genotyping platforms in the last decade has provided scientists with the genomic resources to expedite precise mapping of target loci. Here, we describe methodologies for genetic analysis and application of NGS-based resources to determine the precise genomic locations of rust resistance loci in wheat and development of closely linked markers for marker assisted selection.
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