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A Retrograde Implantation Approach for Peritoneal Dialysis Catheter Placement in Mice
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Liposome-supported enzymatic peritoneal dialysis.
Anna Pratsinis1, Stefanie Zuercher1, Vincent Forster1
1Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, 8093, Zurich, Switzerland.
Biomaterials
|September 1, 2017
Summary
Liposome-supported enzymatic peritoneal dialysis (LSEPD) enhances alcohol metabolism using enzyme-loaded liposomes. This innovative method improves enzyme stability and peritoneal retention for acute alcohol poisoning treatment.
Area of Science:
- Biotechnology
- Biochemistry
- Medical Engineering
Background:
- Peritoneal dialysis is less invasive than hemodialysis but lacks efficacy.
- Acute alcohol poisoning requires effective and rapid detoxification methods.
Purpose of the Study:
- To explore the feasibility of liposome-supported enzymatic peritoneal dialysis (LSEPD) for enhanced xenobiotic metabolism.
- To investigate the performance of enzyme-loaded liposomes (E-Liposomes) for treating acute alcohol poisoning.
Main Methods:
- Development of E-Liposomes containing alcohol oxidase and catalase.
- In vitro and in vivo evaluation of E-Liposomes compared to free enzymes.
- Assessment of LSEPD in a rodent ethanol intoxication model.
Main Results:
- E-Liposomes demonstrated enhanced stability, overcoming thermal instability of alcohol oxidase.
- In vitro ethanol elimination was accelerated by E-Liposomes and hydrogen peroxide.
- LSEPD increased acetaldehyde production in vivo, indicating enhanced ethanol metabolism.
- E-Liposomes showed reduced systemic exposure and organ distribution compared to free enzymes.
Conclusions:
- LSEPD is a feasible platform for temporarily enhancing xenobiotic metabolism.
- Improved enzyme stability and peritoneal retention are key advantages of LSEPD.
- LSEPD offers a promising approach for managing acute alcohol intoxication.
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