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Self-Assembled DNA Generated Electric Current Biosensor for HER2 Analysis.

Congcong Shen1, Ke Zeng1, Junjun Luo1

  • 1College of Chemistry and Chemical Engineering, Central South University , Changsha, China , 410083.

Analytical Chemistry
|September 2, 2017
PubMed
Summary
This summary is machine-generated.

A novel DNA self-assembly amplification technology generates electric current for detecting human epidermal growth factor receptor 2 (HER2). This aptasensor shows high sensitivity and potential for breast cancer diagnostics.

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Area of Science:

  • Biomedical Engineering
  • Molecular Biology
  • Electrochemistry

Background:

  • Detection of human epidermal growth factor receptor 2 (HER2) is crucial for breast cancer diagnosis and treatment monitoring.
  • Existing biosensing methods may lack sensitivity or require complex procedures.
  • DNA-based technologies offer advantages in amplification and signal generation for biosensing.

Purpose of the Study:

  • To develop and validate a new DNA self-assembly amplification technology for electrochemical biosensing of HER2.
  • To evaluate the sensitivity and specificity of the developed aptasensor.
  • To assess the potential clinical applicability of the biosensor for breast cancer patient monitoring.

Main Methods:

  • Development of a DNA self-assembly amplification strategy using aptamers for HER2 recognition and signal generation.
  • Construction of a sandwich-format aptasensor on an electrode surface.
  • Initiation of auxiliary DNA self-assembly by a DNA primer on the HER2 aptamer.
  • Generation of electrochemical current upon reaction with molybdate.
  • Validation of the aptasensor by comparing results with ELISA measurements in serum samples from breast cancer patients and healthy individuals.

Main Results:

  • The aptasensor with DNA self-assembly demonstrated enhanced sensitivity compared to aptasensors without self-assembly.
  • Aptasensor analysis of HER2 levels in serum showed high correlation (R² = 0.9924) with ELISA measurements.
  • Significantly higher HER2 levels were observed in breast cancer patients compared to healthy individuals.
  • HER2 levels decreased after surgery in patients, indicating dynamic changes.

Conclusions:

  • The developed DNA self-assembly amplification technology provides a sensitive and reliable method for HER2 detection.
  • The aptasensor exhibits potential for non-invasive breast cancer diagnosis and monitoring.
  • This DNA-based electrochemical biosensing approach holds promise for broad biomedical applications in protein detection.