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The stem cell regulator PEDF is dispensable for maintenance and function of hematopoietic stem cells

Emma Rörby1, Matilda Billing1, Maria Dahl1

  • 1Department of Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University Hospital, 22184, Lund, Sweden.

Scientific Reports
|September 2, 2017
PubMed

Insights

Pigment epithelium derived factor (PEDF) is highly expressed in hematopoietic stem cells (HSCs). However, PEDF knockout mice show normal hematopoiesis and regeneration, indicating PEDF is dispensable for HSC function.

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Pigment epithelium derived factor (PEDF) is a secreted glycoprotein with known roles in neural and embryonic stem cell regulation.
  • PEDF is highly expressed in murine long-term hematopoietic stem cells (HSCs).

Purpose of the Study:

  • To investigate the role of PEDF in hematopoietic stem cell (HSC) function and regulation.
  • To determine if PEDF is critical for hematopoiesis and stem cell regeneration in vivo and in vitro.

Main Methods:

  • Analysis of PEDF expression in murine HSC compartments.
  • Characterization of the hematopoietic system in PEDF knockout mice.
  • Serial competitive transplantation assays in mice.
  • In vitro culture of human cord blood stem and progenitor cells with recombinant PEDF.

Main Results:

  • PEDF is highly expressed in primary long-term murine HSCs.
  • PEDF knockout mice exhibit normal hematopoiesis under steady-state conditions.
  • Absence of PEDF does not impair hematopoietic regeneration capacity in serial transplantation.
  • PEDF deficiency does not alter lineage distribution upon serial transplantation.
  • Recombinant PEDF does not enhance the growth potential of human cord blood stem and progenitor cells in vitro.

Conclusions:

  • PEDF is not a critical regulatory factor for murine hematopoietic stem cell (HSC) function.
  • PEDF dispensable for HSC regulation during regeneration in vivo.
  • PEDF does not influence the growth of human stem and progenitor cells in vitro.

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