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Ice nucleating agents allow embryo freezing without manual seeding.

Magda Teixeira1, Samuel Buff1, Hugo Desnos2

  • 1Univ Lyon, VetAgro Sup, Marcy l'Etoile, France.

Theriogenology
|September 2, 2017
PubMed
Summary
This summary is machine-generated.

Snomax, an ice nucleating agent, effectively replaces manual seeding in slow freezing protocols for mouse embryos. This method ensures consistent embryo cryopreservation and development without altering medium properties.

Keywords:
AnimalsCryopreservation/methodsCrystallizationEmbryonic developmentFreezingIce nucleating agentKineticsSnomax

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Area of Science:

  • Cryobiology
  • Developmental Biology
  • Biotechnology

Background:

  • Manual seeding is a critical but challenging step in slow embryo freezing.
  • Standardization and sample accessibility issues limit manual seeding in certain cryopreservation systems.

Purpose of the Study:

  • To evaluate Snomax as an alternative ice nucleating agent to manual seeding for mouse embryo cryopreservation.
  • To assess Snomax's impact on freezing medium properties and embryo viability.

Main Methods:

  • Assessed Snomax's effect on pH and osmolality of freezing media.
  • Evaluated in vitro development (blastocyst formation, hatching) of fresh and cryopreserved embryos.
  • Measured mitochondrial activity in frozen-thawed blastocysts using JC-1 staining.

Main Results:

  • Snomax did not alter the physicochemical properties of the freezing medium.
  • Exposure to Snomax did not affect the in vitro development of fresh mouse embryos.
  • Cryopreservation using Snomax as an ice nucleating agent yielded comparable embryo development and mitochondrial activity to manual seeding.

Conclusions:

  • Snomax is an effective ice nucleating agent for slow freezing of mouse embryos.
  • Snomax offers a viable alternative to manual seeding, especially in research settings where manual seeding is impractical (e.g., DSC, cryomicroscopy).