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Updated: Feb 23, 2026
Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine
Published on: October 11, 2019
LC-MS/MS quantitation of antimalarial drug piperaquine and metabolites in human plasma
Mohd Yusmaidie Aziz1, Kurt-Jürgen Hoffmann2, Michael Ashton2
1Unit for Pharmacokinetics and Drug Metabolism, Dept. Pharmacology, Sahlgrenska Academy at University of Gothenburg, Sweden; Advanced Medical and Dental Institute, Universiti Sains Malaysia (USM), Malaysia.
Purpose:
This study aimed to develop a sensitive, quantitative assay for the antimalarial piperaquine (PQ) and its metabolites M1 and M2 in human plasma.
Results:
Analytes were gradiently separated on a C18 column and detected with a Sciex API 4000 MS/MS with an ESI source operated in the positive ion mode with deuterated PQ as internal standard. The response was linear in the range 3.9-2508nM with a runtime of 7.0min per sample. The method was applied to clinical samples from healthy volunteers.
Conclusion:
This LC-MS/MS method for the simultaneous quantitation of PQ and two of its metabolites in plasma may prove helpful for assessment of metabolite safety issues in vivo.
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