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Updated: Feb 23, 2026
Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos
Published on: June 12, 2020
Window of implantation transcriptomic stratification reveals different endometrial subsignatures associated with live
Patricia Díaz-Gimeno1, Maria Ruiz-Alonso2, Patricia Sebastian-Leon1
1Fundacion IVI-Instituto Universitario IVI, University of Valencia, Valencia, Spain; Instituto de Investigación Sanitaria INCLIVA, Valencia, Spain.
Objective:
To refine the endometrial window of implantation (WOI) transcriptomic signature by defining new subsignatures associated to live birth and biochemical pregnancy.
Design:
Retrospective cohort study.
Setting:
University-affiliated in vitro fertilization clinic and reproductive genetics laboratory.
Patient(S):
Healthy fertile oocyte donors (n = 79) and patients with infertility diagnosed by Endometrial Receptivity Analysis (n = 771).
Intervention(S):
None.
Main Outcome Measure(S):
WOI transcriptomic signatures associated with specific reproductive outcomes.
Result(S):
The retrospective cohort study was designed to perform a prediction model based on transcriptomic clusters for endometrial classification (training set, n = 529). The clinical follow-up set in the expected WOI (n = 321) was tested with the transcriptomic predictor to detect WOI variability and the pregnancy outcomes associated with these subsignatures (n = 228). The endometrial receptivity signature was redefined into four WOI transcriptomic profiles. This stratification identified an optimal endometrial receptivity (RR) signature resulting in an ongoing pregnancy rate (OPR) of 80% in terms of live birth, as well as a late receptive-stage (LR) signature with a potential high risk of 50% biochemical pregnancy. Abnormal down-regulation of the cell cycle was the main dysregulated function among the 22 genes associated with biochemical pregnancy.
Conclusion(S):
The major differences between the WOI transcriptomic stratification were in the OPR and biochemical pregnancy rate. The OPR ranged from 76.9% and 80% in the late prereceptive (LPR) and RR signatures, respectively, versus 33.3% in the LR. The biochemical pregnancy rate was 7.7% and 6.6% in LPR and RR, respectively, but 50% in LR, which highlights the relevance of endometrial status in the progression of embryonic implantation.
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