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Nitric oxide sensing by chlorophyll a.

Abhishek Bhattacharya1, Pranjal Biswas1, Puranjoy Kar1

  • 1Department of Biochemistry, University of Calcutta 35, Ballygunge Circular Road, Kolkata 700019, India.

Analytica Chimica Acta
|September 3, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel method using chlorophyll to detect and differentiate various nitric oxide (NO) species in plants. This breakthrough offers a simple, sensitive tool for studying NO signaling in the plant kingdom.

Keywords:
Chlorophylla (Chla)CyanobacteriaNear infra-red fluorescence (NIRF)Nitric oxide (NO)Nitrite (NO(2)(−))S-Nitroso-glutathione (SNO)

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Area of Science:

  • Biochemistry
  • Plant Science
  • Analytical Chemistry

Background:

  • Nitric oxide (NO) is a crucial signaling molecule with largely unexplored roles in plants.
  • Sensing and classifying diverse NO species in biological systems remains a significant challenge.
  • Existing probes for NO detection are limited in their ability to differentiate various NO forms.

Purpose of the Study:

  • To propose a simple and effective method for sensing and classifying different nitric oxide (NO) species.
  • To utilize chlorophyll as a sensing agent, inspired by hemoglobin's role in mammalian systems.
  • To investigate the potential of chlorophyll-based sensing for NO species in the plant kingdom.

Main Methods:

  • Development of a spectroscopic method using chlorophyll a (chl a) for NO species detection.
  • Classification of NO, DETA-NONOate, nitrite, nitrate, and S-nitrosothiol (SNO) using chl a.
  • Molecular docking simulations to understand NO and SNO binding with chlorophyll; experimental validation.
  • Application of the sensing module in microscopic inspection of NO species in cyanobacteria.
  • Investigation of temperature and static magnetic field effects on NO binding with chlorophyll.

Main Results:

  • The proposed method successfully discriminates between various NO species with nano molar (nM) sensitivity at temperatures between 293 K and 310 K.
  • Molecular docking results align with experimental observations, confirming differential binding of NO and SNO with chlorophyll.
  • The chlorophyll-based sensing module demonstrated utility in microscopic analysis of NO species in diverse cyanobacteria.
  • NO binding sensitivity to temperature and magnetic fields supports the involvement of porphyrin ring structures in the sensing mechanism.

Conclusions:

  • Chlorophyll a provides a sensitive and discriminative platform for detecting various nitric oxide (NO) species.
  • The method offers a novel approach for studying NO signaling in plants, overcoming previous sensing limitations.
  • The findings broaden the scope of NO sensing applications, including microscopic imaging and exploring temperature/magnetic field influences.