De novo root regeneration from leaf explants: wounding, auxin, and cell fate transition
1National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
Abstract:
Root organogenesis after tissue damage is a type of plant regeneration known as de novo root regeneration (DNRR). The DNRR process is widely exploited in agricultural technologies, such as cuttings for vegetative propagation. This review summarizes recent advances in our understanding of the cellular and molecular framework of DNRR, mainly focusing on rooting from Arabidopsis thaliana leaf explants. The framework comprises three successive phases, that is, early signaling, auxin accumulation, and cell fate transition, and involves two types of cells with different functions: the converter cell that converts the early signals as the input into auxin flux as the output; and the regeneration-competent cell that undergoes fate transition guided by auxin.
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