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MiR-542-3p exerts tumor suppressive functions in non-small cell lung cancer cells by upregulating FTSJ2

Bin Liu1, Jing Li2, Min Zheng1

  • 1Department of Medical Oncology, Sichuan Cancer Hospital, Chengdu, China.

Life Sciences
|September 4, 2017
PubMed
Abstract

Insights

MicroRNA-542-3p acts as a tumor suppressor in non-small cell lung cancer (NSCLC) by targeting FTSJ2. This finding suggests miR-542-3p and FTSJ2 could be biomarkers for NSCLC treatment response.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • MicroRNAs (miRNAs) play crucial roles in cancer development, with altered expression observed in non-small cell lung cancer (NSCLC).
  • The specific function and regulatory mechanisms of miR-542-3p in NSCLC remain largely unelucidated.
  • Understanding miRNA involvement is key to developing novel therapeutic strategies for NSCLC.

Purpose of the Study:

  • To investigate the biological function of miR-542-3p in NSCLC tumorigenesis.
  • To elucidate the regulatory relationship between miR-542-3p and FTSJ2 in NSCLC.
  • To assess the potential of miR-542-3p and FTSJ2 as predictive biomarkers for NSCLC treatment.

Main Methods:

  • Down-regulation of miR-542-3p in NSCLC tissues and cells was confirmed.
  • Functional assays (MTT, colony formation, Transwell, flow cytometry) assessed the impact of miR-542-3p overexpression on NSCLC cell behavior.
  • EGFP reporter assays, RT-qPCR, and Western blot analyses were employed to determine the direct interaction between miR-542-3p and FTSJ2, and their expression levels.

Main Results:

  • Overexpression of miR-542-3p significantly inhibited NSCLC cell proliferation, migration, cell cycle progression, epithelial-mesenchymal transition (EMT), and tumor growth in vitro and in vivo.
  • miR-542-3p directly targets the 3'UTR of FTSJ2, leading to the upregulation of FTSJ2 at both mRNA and protein levels.
  • FTSJ2 was found to reduce the aggressiveness of NSCLC cells.

Conclusions:

  • miR-542-3p functions as a tumor suppressor gene in NSCLC by targeting and upregulating FTSJ2, thereby inhibiting cancer cell malignancy.
  • miR-542-3p and its target FTSJ2 hold potential as indispensable predictive biomarkers for treatment response in NSCLC patients.
Keywords:
EMTFTSJ2NSCLCUp-regulatedmiR-542-3p

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