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Updated: Feb 23, 2026
Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
A thermostable pyrimidine nucleoside phosphorylase from Brevibacillus borstelensis LK01 for synthesizing halogenated
Ke Liu1, Youzhi Zhou1, Jinsong Zhang1
1College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
Objective:
To isolate a thermostable pyrimidine nucleoside phosphorylase (PyNP) from mesophilic bacteria by gene mining.
Results:
BbPyNP from Brevibacillus borstelensis LK01 was isolated by gene mining. BbPyNP had a highest 60% identity with that of reported PyNPs. BbPyNP could catalyze the phosphorolysis of thymidine, 2'-deoxyuridine, uridine and 5-methyuridine. BbPyNP had good thermostability and retained 73% of its original activity after 2 h incubation at 50 °C. BbPyNP had the highest activity at an optimum alkaline pH of 8.5. BbPyNP was stable from pH 7 to 9.8. Under preliminary optimized conditions, the biosynthesis of various 5-halogenated pyrimidine nucleosides by BbPyNP reached the yield of 61-84%.
Conclusion:
An efficient approach was estimated in isolating thermostable PyNP from mesophilic bacteria.
