Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Caspases01:24

Caspases

14.3K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
14.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acetylation-dependent regulation of FOX-Ku interaction by GCN5-SIRT1 axis controls non-homologous end-joining DNA repair.

Archives of pharmacal research·2026
Same author

Polymerized Polyphenol-Decorated Cationic Hydroxyethylcellulose Nanocomplexes for Enhanced Breast Cancer Cell Killing Effects.

Biomacromolecules·2026
Same author

Structure-dependent inhibition of human carboxylesterase 1 by organophosphate esters: integrated in vitro profiling and 3D-QSAR interpretation.

Chemico-biological interactions·2026
Same author

Polyethyleneimine-functionalized MXene as stable two-dimensional platforms for gene delivery systems.

Chemical communications (Cambridge, England)·2026
Same author

A <i>meso</i>-acyl-BODIPY-based fluorogenic probe for the selective monitoring of aldo-keto reductase 1C1 in living cells.

Chemical communications (Cambridge, England)·2026
Same author

Low-dose radiation generated ROS-activatable doxorubicin prodrug loaded liposome nanoparticles for triple-negative breast cancer treatment.

Journal of controlled release : official journal of the Controlled Release Society·2026

Related Experiment Video

Updated: Feb 23, 2026

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
08:47

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Published on: March 5, 2018

9.5K

Excimer Emission-Based Fluorescent Probe Targeting Caspase-3.

Tae-Il Kim1, Hanyong Jin2, Jeehyeon Bae2

  • 1Department of Chemistry and Research Institute of Basic Sciences, Kyung Hee University , 126 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea.

Analytical Chemistry
|September 6, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel fluorescent probe to detect caspase-3 activity. This probe enables visualization of enzyme activity in vitro and in living cells through enhanced red fluorescence upon aggregation.

More Related Videos

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry
05:29

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Published on: March 24, 2023

5.6K
In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
13:21

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Published on: November 27, 2016

9.6K

Related Experiment Videos

Last Updated: Feb 23, 2026

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
08:47

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Published on: March 5, 2018

9.5K
Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry
05:29

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Published on: March 24, 2023

5.6K
In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
13:21

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

Published on: November 27, 2016

9.6K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Apoptotic protease caspase-3 plays a critical role in programmed cell death.
  • Monitoring caspase-3 activity is crucial for understanding cellular processes and disease states.
  • Existing methods for caspase-3 detection may have limitations in sensitivity or applicability to living systems.

Purpose of the Study:

  • To develop a novel fluorescent probe for sensitive detection and imaging of caspase-3 activity.
  • To utilize an excimer-forming benzothiazolyl-cyanovinylene (CV) dye for enhanced fluorescence signaling.
  • To enable both in vitro studies and in vivo imaging of caspase-3 activity in living cells.

Main Methods:

  • Synthesis of a water-soluble fluorescent probe (Ac-DEVD-NH-CV) targeting caspase-3.
  • Enzymatic cleavage of the probe by caspase-3 to yield a hydrophobic, aggregating dye (CV-NH2).
  • Utilizing the aggregation-induced excimer emission of the CV dye for signal amplification.

Main Results:

  • The probe is converted from a weakly green emissive form to a strongly red-shifted and enhanced fluorescent species upon caspase-3 action.
  • The hydrophobic product (CV-NH2) spontaneously aggregates, promoting excimer formation and signal enhancement.
  • Successful application of the probe for studying caspase-3 activity in vitro and imaging its activity in living cells.

Conclusions:

  • The developed fluorescent probe offers a sensitive and effective tool for monitoring caspase-3 activity.
  • The probe's design allows for ratiometric and amplified fluorescence detection, suitable for biological imaging.
  • This technology has potential applications in cell biology research and diagnostics related to apoptosis.