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Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
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Endodermal germ-layer formation through active actin-driven migration triggered by N-cadherin.

Florence A Giger1,2,3, Nicolas B David4,2,3,5

  • 1CNRS UMR8197, F-75005 Paris, France.

Proceedings of the National Academy of Sciences of the United States of America
|September 7, 2017
PubMed
Summary
This summary is machine-generated.

Germ-layer formation involves active cell migration, not just differential adhesion. Endodermal cells move away from neighbors, driven by N-cadherin, to reach their internal position during zebrafish gastrulation.

Keywords:
cadherincell migrationendodermgastrulationzebrafish

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Embryogenesis

Background:

  • Germ-layer formation is crucial for development and tissue remodeling.
  • The cellular and molecular mechanisms of germ-layer segregation remain poorly understood due to limited in vivo observation.
  • Existing models often emphasize differential cell adhesion for tissue patterning.

Purpose of the Study:

  • To investigate the cellular and molecular basis of endoderm formation during zebrafish gastrulation.
  • To elucidate the mechanisms driving germ-layer segregation in vivo.
  • To challenge and refine existing models of tissue formation.

Main Methods:

  • Utilized mosaic zebrafish embryos for in vivo studies.
  • Employed high-resolution live imaging to observe cell behavior.
  • Conducted functional analyses, including manipulating N-cadherin expression.

Main Results:

  • Endodermal cells actively migrate to their innermost position via an oriented, actin-based process dependent on Rac1.
  • Observed cell-contact avoidance, where cells migrate away from neighbors, rather than attraction to the yolk syncytial layer.
  • Demonstrated that N-cadherin expression in ectodermal cells can induce their internalization without altering cell fate.

Conclusions:

  • Germ-layer formation is driven by active cell migration and cell-contact avoidance, challenging the differential adhesion model.
  • N-cadherin plays a key role in mediating this outward migration of endodermal cells.
  • Cell-contact avoidance represents a novel, unexplored mechanism in germ-layer formation.