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Updated: Feb 23, 2026

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Published on: March 28, 2025

Functional vascularized lung grafts for lung bioengineering.

N Valerio Dorrello^1,2, Brandon A Guenthart^2,3, John D O'Neill²

¹Department of Pediatrics, Columbia University Medical Center, New York, NY 10032, USA.

Science Advances

|September 7, 2017

Summary

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Researchers developed a novel airway-specific method to remove lung epithelium while preserving vasculature. This technique supports cell growth on lung grafts, paving the way for bioengineered lungs and improved lung repair therapies.

Area of Science:

Biomedical Engineering
Regenerative Medicine
Pulmonary Biology

Background:

End-stage lung disease is a major global health concern, necessitating innovative therapeutic strategies.
Previous lung bioengineering efforts failed due to the inability to maintain a functional vascular network.
An intact vasculature is crucial for graft survival, blood-gas exchange, and regenerative cell support.

Purpose of the Study:

To develop a method for bioengineering functional lungs for transplantation.
To investigate an airway-specific approach to lung tissue regeneration.
To assess the viability of preserving vascular endothelium during lung de-epithelialization.

Main Methods:

Developed an airway-specific protocol to remove pulmonary epithelium in a rat model.

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Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

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Procedure for Lung Engineering

Procedure for Lung Engineering

Published on: March 8, 2011

Related Experiment Videos

Last Updated: Feb 23, 2026

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Published on: March 28, 2025

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

Procedure for Lung Engineering

Procedure for Lung Engineering

Published on: March 8, 2011

Focused on maintaining the viability and function of the vascular endothelium.

Utilized the resulting vascularized lung grafts to support cell attachment and growth.

Main Results:

Successfully removed pulmonary epithelium while preserving vascular integrity.
Demonstrated that vascularized lung grafts supported human adult pulmonary cells.
Showed support for stem cell-derived lung-specified epithelial cells on the grafts.

Conclusions:

Lung de-epithelialization preserving vasculature is a viable strategy for lung bioengineering.
This approach facilitates cell therapy for pulmonary epithelium regeneration.
Enables the potential for creating functional bioengineered lungs for transplantation.