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Published on: June 28, 2019
Production of tartaric acid using immobilized recominant cis-epoxysuccinate hydrolase
Ziqiang Wang1, Munan Su1, Yanliang Li1
1State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.
Objective:
To investigate the expression and immobilization of recombinant cis-epoxysuccinate hydrolase (ESH), and its application in the biological production of L-(+)-tartaric acid.
Results:
E. coli BL21 (DE3)/pET11a-ESH (His) was engineered to express recombinant ESH. The enzyme had an activity of 262 U mg-1. The recombinant ESH was immobilized on agarose Ni-IDA matrix with metal ion affinity interaction to improve its thermostability and pH stability. The immobilization efficiency and activity yield were 94 and 95%, respectively. The specific catalytic efficiency of immobilized ESH was 104 mg U-1 h-1 during the continuous enzymatic production process.
Conclusion:
ESH with a histidine tag was immobilized and used for the continuous production of L-(+)-tartaric acid.
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