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Related Experiment Video

Updated: Feb 23, 2026

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Integrated Three-Dimensional Microanalysis Combining X-Ray Microtomography and X-Ray Fluorescence Methodologies.

Brecht Laforce1, Bert Masschaele2,3, Matthieu N Boone2

  • 1X-ray Microspectroscopy and Imaging group (XMI), Department of Analytical Chemistry, Ghent University , Krijgslaan 281 S12, B-9000 Ghent, Belgium.

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|September 7, 2017
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Summary

A new instrument, Herakles, integrates X-ray computed tomography (μCT), X-ray fluorescence tomography (XRF-CT), and confocal XRF for 3D elemental and morphological analysis. This provides unprecedented detail linking chemical composition to structure in materials.

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Advanced materials characterization requires correlating elemental distribution with 3D morphology.
  • Existing laboratory X-ray techniques have limitations in achieving this integrated analysis at high resolution.

Purpose of the Study:

  • To introduce a novel integrated laboratory instrument (Herakles) for simultaneous 3D elemental and morphological analysis.
  • To demonstrate the capability of linking elemental distribution with 3D structure at micrometer resolution.

Main Methods:

  • Combining X-ray computed tomography (μCT), X-ray fluorescence tomography (XRF-CT), and confocal XRF within a single instrument.
  • Utilizing a high-precision air-bearing motor system for seamless integration and coordinate system retention.
  • Employing in-house developed control and analysis software for integrated workflow.

Main Results:

  • Achieved 1-10 μm resolution for combined elemental and morphological analysis.
  • Demonstrated quantitative spatial distribution of chemical elements linked to 3D internal structure.
  • Case studies on a Cu test pattern, Daphnia magna, and perlite biocatalyst support validated instrument performance.

Conclusions:

  • The Herakles instrument offers a state-of-the-art solution for high-resolution 3D elemental and morphological analysis.
  • Integration of μCT, XRF-CT, and confocal XRF provides complementary insights not achievable with individual techniques.
  • The developed approach advances laboratory X-ray analysis capabilities for diverse scientific investigations.