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Updated: Feb 23, 2026

Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows
Published on: April 25, 2013
Label-Free Transient Absorption Microscopy for Red Blood Cell Flow Velocity Measurement in Vivo
Tao Chen1, Yanyi Huang1,2
1Biodynamic Optical Imaging Center (BIOPIC), and College of Engineering, Peking University , Beijing 100871, China.
Abstract:
Red blood cells have intrinsic transient absorption property at the near-infrared region, allowing for label-free detection and imaging. We present a new approach to measure the blood flow velocity in vivo with a transient absorption microscope and correlation analyses of signal sequences. With specific scan modes, we have quantitatively obtained the flow velocity in capillaries, arteries, and veins in a live zebrafish with accuracy of about 30 μm/s. In addition, a high-resolution three-dimensional vessel network can be reconstructed through this approach with spatial resolution of 1 μm.

