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Published on: May 25, 2018
Establishment of a mouse xenograft model of metastatic adrenocortical carcinoma
Aurélie Morin1,2, Carmen Ruggiero3,4, Estelle Robidel1,2
1Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
Abstract:
Adrenocortical carcinoma is a rare neoplasm with a poor prognosis. Very important advances have been made in the identification of the genetic determinants of adrenocortical carcinoma pathogenesis but our understanding is still limited about the mechanisms that determine cancer spread and metastasis. One major problem hindering preclinical experimentation for new therapies for adrenocortical carcinoma is represented by the lack of suitable animal models for metastatic disease. With the aim to overcome these limitations, in this study we tested several protocols in order to establish a mouse xenograft model of metastatic adrenocortical carcinoma. The most efficient method, based upon intrasplenic injection followed by splenectomy, produced metastases with high efficiency, whose development could be followed over time by bioluminescence measurements. We expect that the availability of this model will greatly improve the possibilities for preclinical testing of new treatments for advanced-stage disease.
Insights
Researchers developed a new mouse model for metastatic adrenocortical carcinoma (ACC). This model efficiently creates metastases, aiding the preclinical testing of novel therapies for advanced ACC.
Area of Science:
- Oncology
- Cancer Biology
- Translational Research
Background:
- Adrenocortical carcinoma (ACC) is a rare cancer with a poor prognosis.
- Understanding metastasis mechanisms in ACC is limited.
- Lack of suitable animal models hinders preclinical therapy testing.
Purpose of the Study:
- To establish a mouse xenograft model for metastatic adrenocortical carcinoma.
- To overcome limitations in preclinical experimentation for ACC therapies.
Main Methods:
- Tested several protocols to establish a metastatic ACC mouse model.
- Utilized intrasplenic injection followed by splenectomy.
- Monitored metastasis development using bioluminescence imaging.
Main Results:
- The intrasplenic injection and splenectomy method efficiently produced metastases.
- The model allows for longitudinal monitoring of metastatic disease.
- High efficiency in establishing metastatic disease was achieved.
Conclusions:
- A novel and efficient mouse xenograft model for metastatic adrenocortical carcinoma has been established.
- This model will facilitate preclinical testing of new treatments for advanced ACC.
- The model improves research capabilities for understanding ACC metastasis.
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