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Clickable Multifunctional Dumbbell Particles for in Situ Multiplex Single-Cell Cytokine Detection
Peng Zhao1, Justin George1, Bin Li1
1Multiplex Biotechnology Laboratory, Department of Chemistry, University at Albany, State University of New York , Albany, New York 12222, United States.
ACS Applied Materials & Interfaces
|September 9, 2017
Summary
Researchers developed novel multifunctional dumbbell particles (DPs) using click chemistry for monitoring single-cell cytokine release. These particles enable precise cell capture and multiplex cytokine detection, advancing single-cell analysis and diagnostics.
Area of Science:
- Biotechnology
- Chemical Biology
- Single-cell analysis
Background:
- Monitoring cytokine release from single cells is crucial for understanding cellular responses.
- Existing methods for single-cell cytokine analysis face limitations in sensitivity and multiplexing capabilities.
Purpose of the Study:
- To develop a novel strategy for fabricating multifunctional dumbbell particles (DPs) for monitoring single-cell cytokine releasing.
- To functionalize DPs for efficient cell capturing and multiplex cytokine detection.
Main Methods:
- Fabrication of multifunctional dumbbell particles (DPs) using click chemistry via covalent bioorthogonal reactions (methyltetrazine and trans-cyclooctene) on a microchip under a magnetic field.
- Functionalization of DPs with antibodies for specific cell capture and cytokine detection.
- Utilizing fluorescently labeled DPs for multiplex detection and analysis of cytokines secreted by single live cells.
Main Results:
- Successfully prepared multifunctional dumbbell particles (DPs) on a large scale.
- Demonstrated the ability of DPs to capture cells and detect multiple cytokines simultaneously from single live cells.
- DPs labeled with different fluorescent dyes enabled multiplex cytokine analysis.
Conclusions:
- The developed multifunctional dumbbell particles (DPs) offer a promising new tool for single-cell cytokine monitoring.
- These DPs have potential applications in cell sorting, bioimaging, single-cell analysis, and biomedical diagnostics.
