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RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis

Jessie M Sutherland1,2, Alexander P Sobinoff2,3, Barbara A Fraser2

  • 1School of Biomedical Science & Pharmacy, University of Newcastle, Callaghan, Australia.

Journal of Cellular Physiology
|September 9, 2017
PubMed

Insights

Overexpressing Musashi-2 (MSI2) in male germ cells disrupts sperm development. MSI2 targets key genes like Tbx1 and Piwil1, impacting cell cycle and fertility.

Area of Science:

  • Reproductive biology
  • Molecular genetics
  • Gene regulation

Background:

  • RNA-binding proteins (RBPs) regulate gene expression post-transcriptionally.
  • Nuclear overexpression of Musashi-2 (MSI2) impairs male germ cell maturation and fertility.
  • Understanding MSI2's targets is crucial for male reproductive health.

Purpose of the Study:

  • To identify genes and pathways affected by MSI2 upregulation during male gamete development.
  • To elucidate the mechanisms by which MSI2 impacts spermatogenesis.

Main Methods:

  • Microarray analysis and quantitative PCR (qPCR) for gene expression profiling.
  • Isobaric tag for relative and absolute quantitation (iTRAQ), immunoblotting, and immunolocalization for protein analysis.

Main Results:

  • Differential gene expression observed in cell cycle, proliferation, and apoptosis pathways.
  • Altered expression and localization of transcription, translation, RNA processing, and spermatogenesis regulators.
  • MSI2 identified as a repressor of Tbx1 and Piwil1 translation.

Conclusions:

  • MSI2 plays a critical role in post-transcriptional gene regulation during male gamete development.
  • MSI2's impact on Tbx1 and Piwil1 highlights its significance in spermatogenesis.
  • This study provides evidence for MSI2 as a key regulator of male fertility.
Keywords:
MSI2RBPgametogenesisspermatidtestes

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