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Updated: Feb 23, 2026

Extraction of Saliva, Haemolymph, Salivary Glands, and Midgut from Individual Ticks (Acari: Ixodidae)
Published on: October 31, 2025
Microbial diversity of the Tibetan tick Haemaphysalis tibetensis (Acari: Ixodidae)
Zhijun Yu1, Rongrong Wang1, Ningxin Li1
1Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China.
Abstract:
The Tibetan tick Haemaphysalis tibetensis is widely distributed in the Tibetan Plateau, and is recognized as one of the primary parasites affecting domestic and wild animals. No information is available on its microbial diversity. In the current study, the microbiomes in H. tibetensis were explored using Illumina MiSeq sequencing. Results indicate that a total of 28 phyla and 38 genera were characterized from this tick, and most of the microbes were classified in the phylum Proteobacteria. Female and male H. tibetensis harbored similar composition of the microbes, with dominant bacteria from the genus Coxiella accounting for 87.5 and 66.5% in females and males, respectively. The abundance of bacteria belonging to the genus Rickettsia in females and males was 6.9 and 23.7%, respectively. These findings may help understand the relationship between this tick and its symbiontic microorganisms.
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