Chassis and key enzymes engineering for monoterpenes production

Area of Science:

• Microbial biotechnology
• Metabolic engineering
• Synthetic biology

Background:

• Microbial monoterpene production is hindered by product cytotoxicity and metabolic instability.
• Key enzymes and chassis properties significantly impact production efficiency.

Purpose of the Study:

• To review recent advancements in microbial monoterpene production.
• To highlight strategies for overcoming production limitations.

Main Methods:

• Review of literature on cytotoxicity alleviation in microbial chassis.
• Analysis of methods for reducing in vivo conversion of monoterpenes.
• Examination of enzyme engineering approaches, including geranyl diphosphate synthase and monoterpene synthases.

Main Results:

• Engineering strategies can significantly improve microbial tolerance to monoterpenes.
• Metabolic engineering approaches can reduce undesirable in vivo conversions.
• Enzyme engineering enhances the efficiency of monoterpene biosynthesis pathways.

Conclusions:

• Development of robust microbial chassis with enhanced cytotoxicity tolerance is crucial.
• Tailoring metabolic pathways and enzyme kinetics improves monoterpene yield, titer, and productivity.
• These advancements pave the way for efficient microbial production of valuable monoterpenes.