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Quantitative RNA Analysis from Urine Using Real Time PCR
Lourdes Mengual1, Mireia Olivan2
1Laboratory and Department of Urology, Centre de Recerca Biomèdica CELLEX, Hospital Clínic, Universitat de Barcelona, IDIBAPS, C/Casanova, 143, Barcelona, 08036, Barcelona, España. lmengual@clinic.ub.es.
Methods in Molecular Biology (Clifton, N.J.)
|September 11, 2017
Summary
Urine analysis offers a minimally invasive method for cancer detection. This study details a protocol for isolating urinary cells and quantifying their gene expression for early cancer diagnosis.
Area of Science:
- Urology
- Oncology
- Molecular Biology
Background:
- Urine is a promising biological fluid for non-invasive liquid biopsies.
- Analyzing urinary biomarkers aids in cancer prevention and early detection.
- Urothelial carcinoma assessment benefits from urinary cell fraction biomarker analysis.
Purpose of the Study:
- To present a protocol for collecting and processing urine samples.
- To describe the isolation of RNA from urinary exfoliated cells.
- To detail the quantification of gene expression in these cells.
Main Methods:
- Urine sample collection and processing for exfoliated cell isolation.
- RNA isolation from urinary cells.
- Quantitative gene expression analysis.
Main Results:
- A standardized protocol for obtaining urinary exfoliated cells was established.
- Methodology for RNA isolation and gene expression quantification was successfully applied.
- This approach enables the analysis of biomarkers in urinary cells.
Conclusions:
- The described protocol facilitates the non-invasive identification of cancer biomarkers.
- This method is particularly promising for urothelial carcinoma detection.
- Gene expression analysis in urinary cells supports early cancer diagnosis.
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