CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation
Yuta Nihongaki1, Yuichi Furuhata2, Takahiro Otabe1
1Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan.
Abstract:
Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0.
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