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Modular flow chamber for engineering bone marrow architecture and function.

Christian A Di Buduo1, Paolo M Soprano1, Lorenzo Tozzi2

  • 1Department of Molecular Medicine, University of Pavia, Pavia, Italy; Biotechnology Research Laboratories, IRCCS San Matteo Foundation, Pavia, Italy.

Biomaterials
|September 13, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel silk-based scaffold to engineer bone marrow, successfully producing functional platelets ex vivo. This system aids in studying compounds affecting platelet formation.

Keywords:
Bone marrowHematopoiesisMegakaryocytePlateletSilk

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Area of Science:

  • Biomaterials Science
  • Hematology
  • Tissue Engineering

Background:

  • Bone marrow supports hematopoiesis for blood cell turnover.
  • Silk fibroin is a promising biomaterial for bone marrow engineering due to its properties.
  • Existing methods lack efficient ex vivo blood cell production models.

Purpose of the Study:

  • To develop a functional ex vivo bone marrow scaffold using silk fibroin.
  • To assess the scaffold's capacity for platelet production and recovery.
  • To validate the model's utility in evaluating compounds affecting platelet formation.

Main Methods:

  • Fabrication of a modular flow chamber with a functionalized silk sponge scaffold.
  • Seeding megakaryocytes onto the silk sponge for platelet generation.
  • Perfusion of the chamber and functional assessment of recovered platelets.
  • Inhibition of AKT signaling to evaluate its impact on platelet production.

Main Results:

  • The silk sponge scaffold supported efficient platelet formation from megakaryocytes.
  • Functional platelets were recovered from the system via perfusion.
  • Inhibition of AKT signaling significantly reduced platelet yield, validating the model.
  • The system demonstrated potential for evaluating drug effects on platelet production.

Conclusions:

  • A novel modular silk-based scaffold was bioengineered for simplified bone marrow applications.
  • The system enables ex vivo blood cell production, specifically functional platelets.
  • This technology offers a valuable platform for studying bone marrow functions and compound effects.