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Updated: Feb 23, 2026
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
Published on: September 25, 2011
Transcriptome-wide identification and competitive disruption of sacum-binding partners in human colorectal cancer
Yinguang Zhang1, Yongwang Zhang2, Yuxiang Zhang3
1Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
Abstract:
Human sacum is regulatory adaptor protein involved in cellular signaling network of colorectal cancer. Molecular evidence suggests that the protein is integrated into oncogenic signaling network by binding to SH3-containing proteins through its proline-rich motifs. In this study, we have performed a transcriptome-wide analysis and identification of sacum-binding partners in the genome profile of human colorectal cancer. The sacum-binding potency of SH3-containing proteins found in colorectal cancer was investigated by using bioinformatics modeling and intermolecular binding analysis. With the protocol we were able to predict those high-affinity domain binders of the proline-rich peptides of human sacum in a high-throughput manner, and to analyze sequence-specific interaction in the domain-peptide recognition at molecular level. Consequently, a number of putative domain binders with both high affinity and specificity were identified, from which the Src SH3 domain was selected as a case study and tested for its binding activity towards the sacum peptides. We also designed two peptide variants that may have potent capability to competitively disrupt sacum interaction with its partners.
Insights
This study identifies human sacum binding partners in colorectal cancer, revealing potential therapeutic targets. We found specific protein interactions that could be disrupted to inhibit cancer signaling.
Area of Science:
- Oncology
- Molecular Biology
- Bioinformatics
Background:
- Human sacum is a regulatory adaptor protein implicated in colorectal cancer signaling.
- Sacum interacts with SH3-containing proteins via its proline-rich motifs, integrating into oncogenic networks.
Purpose of the Study:
- To perform a transcriptome-wide analysis identifying sacum-binding partners in human colorectal cancer.
- To investigate the binding affinity of SH3-containing proteins to sacum using bioinformatics and molecular analysis.
- To identify potential therapeutic peptides that disrupt sacum-protein interactions.
Main Methods:
- Transcriptome-wide analysis for sacum-binding partner identification.
- Bioinformatics modeling and intermolecular binding analysis for SH3-containing proteins.
- High-throughput prediction of domain-peptide binders and sequence-specific interaction analysis.
- Case study using Src SH3 domain and design of peptide variants.
Main Results:
- Identification of numerous high-affinity and specific domain binders for human sacum peptides.
- Validation of binding activity for the Src SH3 domain with sacum peptides.
- Design of two peptide variants with potential to disrupt sacum-protein interactions.
Conclusions:
- Sacum plays a key role in colorectal cancer signaling through interactions with SH3-containing proteins.
- This study provides a method for high-throughput identification of sacum-binding partners.
- Identified peptide variants offer a potential therapeutic strategy for colorectal cancer by disrupting oncogenic signaling.
