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Published on: November 2, 2020
Identification of Genes Differentially Expressed Between Midgestational and Postnatal Mouse Skin Through Suppression
Objective:
The aim of this study was to identify differentially expressed genes between the skin of midgestational (embryonic day [ED] 14) and postnatal mice that might be involved in the wound healing process.
Materials And Methods:
BALB/c mice with dated pregnancy underwent laparotomy and hysterotomy on ED14, and skin from 20 fetuses were harvested. Full-thickness dorsal skin was also harvested from 30 three-month-old postnatal mice. Total ribonucleic acid (RNA) and mRNA were purified side-by-side from the harvested skins. Differentially expressed cDNA fragments were isolated by suppression subtractive hybridization (SSH). The cDNA fragments were subsequently cloned, sequenced, and identified through a Nucelotide Basic Local Alignment Search Tool (BLASTN) search.
Results:
Twenty differentially expressed transcripts were identified, including 18 uniquely expressed in fetal mice skin and 2 from postnatal mice skin. The known genes identified include RPS29, Nedd5, EndoA, TIEG, and eEF-1α, which may play an important role in scarless wound healing of embryonic mice. Two novel genes uniquely expressed in fetal mouse skin were also identified.
Conclusion:
Through SSH, 20 differentially expressed genes in the skin of midgestational and postnatal mice were identified. Two novel genes were identified that were uniquely expressed in the midgestational mouse skin. The authors suggest these genes might be involved in the scarless wound healing process.
