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Updated: Feb 23, 2026
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue
Dun Pan1, Xiaolei Zuo2, Ying Wan3
1Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. pandun@sinap.ac.cn.
Abstract:
In this work, we reported a systematic investigation on the interactions betweenmethylene blue (MB) and surface-confined DNA by using electrochemical methods. Wedemonstrated that the redox potential of MB and binding and dissociation kinetics of MB toDNA differed significantly for single-stranded DNA (ss-DNA) and double-stranded DNA(ds-DNA) immobilized on gold electrodes. This was possibly due to the different bindingmechanism between MB and ss- or ds-DNA. This work might provide useful informationfor developing MB-based sequence-specific electrochemical DNA sensors.
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