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Related Concept Videos

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Related Experiment Video

Updated: Feb 23, 2026

Determination of Self-Incompatibility and Inter-Incompatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
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Comparative transcriptome analysis during early fruit development between three seedy citrus genotypes and their

Shujian Zhang1, Qingchun Shi1, Ute Albrecht1

  • 1U.S. Horticultural Research Laboratory, USDA-ARS, Ft. Pierce, FL 34945, USA.

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|September 15, 2017
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Summary

Researchers identified genes with differential transcript abundance (GDTA) in seedless citrus mutants, revealing key genes involved in seed development. Protease genes were particularly important for seedless fruit formation, offering insights for breeding new seedless varieties.

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Area of Science:

  • Plant Molecular Biology
  • Genomics
  • Citrus Horticulture

Background:

  • Seedless citrus varieties are highly desirable for commercial production.
  • Understanding the genetic basis of seedlessness is crucial for developing improved cultivars.

Purpose of the Study:

  • To identify genes with differential transcript abundance (GDTA) between seedy and seedless citrus fruits during early development.
  • To elucidate the molecular mechanisms underlying seedless fruit development in citrus.

Main Methods:

  • Transcriptome analysis using Affymetrix microarrays on three citrus genotypes and their seedless mutants at three developmental time points.
  • Quantitative PCR (qPCR) for validation of differentially abundant transcripts.
  • Hierarchical clustering analysis to group genes based on expression patterns.

Main Results:

  • 359 to 1077 probe sets showed differential transcript abundance between seedy and seedless fruits across genotypes and time points.
  • 18 probe sets identified as GDTA were validated by qPCR.
  • 14, 9, and 12 genes exhibited consistent abundance ratios across all genotypes at time points 1, 2, and 3, respectively.
  • Genes encoding aspartic and cysteine proteases were among those consistently differentially abundant, suggesting a role in seed abortion.

Conclusions:

  • GDTA analysis provides valuable insights into the genetic regulation of seedless citrus fruit development.
  • Protease genes are potential key players in the process of seed abortion leading to seedless fruits.
  • Findings can inform biotechnological strategies for creating novel seedless citrus cultivars.