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Updated: Feb 23, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Published on: February 17, 2017
Recent advances on the difructose anhydride IV preparation from levan conversion
Hua Hang1,2
1Research Institute of Functional Food, Anhui Normal University, Wuhu, 241000, Anhui Province, People's Republic of China. 2006hanghua@163.com.
Abstract:
Difructose anhydride IV (DFA IV) is a cyclic disaccharide consisting of two fructose residues, which is obtained from levan conversion with levan fructotransferase (LFTase) and rarely found in nature as a low-calorie sugar substitute. Some beneficial effects of DFA IV connected with its consumption have been described. The article reviews the properties and physiological functions of DFA IV, levan conversion, resources and properties of LFTase and the produced methods of DFA IV. LFTase as a relatively novel enzyme and its molecular evolution are discussed as well. The aim is to better understand a novel sugar-substituting sweetener of DFA IV as a food additive.
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