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Mott cells: a model to study immunoglobulin secretion.

A Alanen1, U Pira, A Colman

  • 1Biocenter, University of Basel, Switzerland.

European Journal of Immunology
|November 1, 1987
PubMed
Summary
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Mott cells, defective in immunoglobulin secretion, were studied using hybridoma fusions. Cell fusion partially rescued Ig secretion, suggesting complementation of cellular factors involved in this defect.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Mott cells are plasma cells characterized by defective immunoglobulin (Ig) secretion.
  • This defect manifests as Ig accumulation within the rough endoplasmic reticulum, forming detectable Ig+ intracellular inclusions.

Purpose of the Study:

  • To further investigate the cellular defect in Mott cells responsible for impaired Ig secretion.
  • To explore the potential for complementation of this defect through cell fusion and mRNA injection.

Main Methods:

  • Hybridoma cell lines derived from Mott cells were fused with a kappa-secreting hybridoma (Sp1).
  • Selection methods (hypoxanthine, aminopterin, thymidine, and ouabain) were used to isolate hybrid cell lines expressing various Ig chain combinations.
  • Immunoglobulin secretion and intracellular inclusions were analyzed using metabolic labeling and immunoprecipitation.

Related Experiment Videos

  • Xenopus oocytes were injected with mRNA from Mott cell hybridomas to assess Ig secretion.

    • Main Results:

    • All inclusion-positive hybrid clones expressed both Mott heavy and Mott light chains, with or without Sp1 light chain.
    • Ig secretion was at least partially rescued in all fused clones, including an inclusion-positive clone lacking Sp1 kappa chain.
    • Injection of mRNA from Mott cells into Xenopus oocytes led to the secretion of previously non-secreted Ig.

    Conclusions:

    • Cell fusion can complement cellular factors essential for immunoglobulin secretion in Mott cells.
    • The study demonstrates a potential for rescuing Ig secretion defects through cellular complementation and mRNA-mediated expression.