Different approaches to establish infertile rooster

Area of Science:

• Reproductive Biology
• Animal Science
• Spermatogenesis Research

Background:

• Spermatogonial stem cell (SSC) transplantation requires suppression of endogenous spermatogenesis in recipient males.
• Effective methods for depleting endogenous sperm production are crucial for successful SSC transplantation outcomes.

Purpose of the Study:

• To compare the efficacy of gamma radiation and the alkylating agent busulfan in suppressing spermatogenesis in adult ROSS 308 strain roosters.
• To determine appropriate dosages of gamma radiation and busulfan for infertilizing mature roosters.

Main Methods:

• Adult roosters were treated with two gamma radiation regimes (40Gy and 30Gy) or two busulfan dosages (60mg and 50mg).
• Sperm motility, sperm concentration, seminiferous tubule diameter, and epithelium thickness were assessed.
• Body weight and overall health status were monitored throughout the experimental period.

Main Results:

• Both gamma radiation and busulfan treatments significantly reduced sperm motility and concentration compared to controls.
• Sperm concentration reached zero by the fourth week post-treatment across all experimental groups.
• Histological analysis showed significant reductions in seminiferous tubule diameter and epithelium thickness with both treatments.

Conclusions:

• Both 30Gy gamma radiation (3x10Gy) and 50mg busulfan (30+20mg) are effective in suppressing endogenous spermatogenesis in mature roosters.
• These reduced doses offer a viable option for infertilization, considering the risks associated with higher exposure levels.
• The findings support the use of these methods for preparing recipient roosters for SSC transplantation.