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Fed-Batch CHO Cell Culture for Lab-Scale Antibody Production
Yuzhou Fan1, Daniel Ley1, Mikael Rørdam Andersen2,3
1Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 223, 2800 Kgs, Lyngby, Denmark.
Methods in Molecular Biology (Clifton, N.J.)
|September 19, 2017
Abstract:
Fed-batch culture is the most commonly used upstream process in industry today for recombinant monoclonal antibody production using Chinese hamster ovary (CHO) cells. Developing and optimizing this process in the lab is crucial for establishing process knowledge, which enables rapid and predictable tech-transfer to manufacturing scale. In this chapter, we describe stepwise how to carry out fed-batch CHO cell culture for lab-scale antibody production.