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Updated: Feb 22, 2026

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Effect of High Inspiratory Oxygen Fraction on Endothelial Function in Healthy Volunteers: A Randomized Controlled
Mikkel Hjordt Holm Larsen1, Sarah Ekeloef, Dunja Kokotovic
1From the *Department of Surgery, Center for Surgical Science, Zealand University Hospital, Koege, Denmark; and †Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.
Abstract:
It has been suggested that high inspiratory oxygen concentrations during anesthesia may be associated with higher postoperative mortality due to endothelial dysfunction. A randomized controlled crossover study was conducted with 25 healthy male volunteers. They inhaled an oxygen concentration of 30% and 80%. The endothelial function was assessed using noninvasive digital pulse amplitude tonometry (EndoPAT) supported by endothelial biomarkers. The difference in endothelial function between the 2 treatments was 0.05 (95% confidence interval, -0.36 to 0.27; P = .77). Endothelial biomarkers were unaffected. Inhalation of a high oxygen fraction in healthy volunteers did not result in a significant reduction of endothelial function.
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