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Validation of standards suitable for genome size estimation of fungi
Pedro Talhinhas1, Daniela Tavares2, Ana Paula Ramos1
1LEAF-Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
Journal of Microbiological Methods
|September 20, 2017
Abstract:
Genome size information is fundamental to genome sequencing and may also uncover genomic aspects of evolution. Flow Cytometry, the preferred method for genome size estimation, requires suitable standards. Here we validate Inonotus hispidus, Colletotrichum acutatum and Cenococcum geophilum (41, 68 and 203Mbp), as standards for fungal genome size estimation.
Keywords:
Cenococcum geophilumColletotrichum acutatumDNA standardFlow CytometryGenome sizeInonotus hispidus
