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Updated: Feb 22, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Correlative Single-Molecule Localization Microscopy and Confocal Microscopy.

Christian Soeller^1,2, Yufeng Hou^3,4, Isuru D Jayasinghe⁵

¹Living Systems Institute and Biomedical Physics, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK. c.soeller@exeter.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)

|September 20, 2017

Summary

This summary is machine-generated.

Correlative microscopy combines nanoscale imaging of molecular targets using direct stochastic optical reconstruction microscopy (dSTORM) with conventional confocal microscopy for cellular context in human cardiac tissue.

Keywords:

Antibodies Confocal imaging Correlative imaging Fluorescence Heart Human cardiac tissue Multiscale data Super-resolution microscopy

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Last Updated: Feb 22, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

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Published on: August 2, 2018

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Single-molecule localization microscopy (SMLM) provides nanoscale resolution of molecular targets.
Integrating SMLM with broader cellular context remains a challenge for biological studies.

Purpose of the Study:

To develop a correlative imaging procedure for human cardiac tissue.
To combine nanoscale molecular imaging with diffraction-limited cellular context.

Main Methods:

Correlative imaging of human cardiac tissue.
Nanoscale imaging using direct stochastic optical reconstruction microscopy (dSTORM).
Conventional confocal microscopy for diffraction-limited imaging.

Main Results:

Successful implementation of a correlative imaging workflow.
Acquisition of nanoscale dSTORM data alongside confocal microscopy data.
Demonstration of combined high-resolution and contextual imaging in cardiac tissue.

Conclusions:

Correlative microscopy enhances biological insights by integrating molecular and cellular information.
This approach provides valuable context for nanoscale observations in complex tissues.
The described procedure is applicable to studying molecular organization in human cardiac tissue.