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Updated: Feb 22, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
A CRISPR reimagining: New twists and turns of CRISPR beyond the genome-engineering revolution
Robert J Plummer1, Yi Guo1,2, Ying Peng1
1Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota.
Abstract:
Despite its explosive applications in genome engineering, CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) has been developed into a versatile tool beyond its well-known nuclease function. In this prospect article, we summarize a few exciting "off-label" applications of CRISPR including manipulating DNA sequences, visualizing chromosomal loci in living cells, and modulating transcription and chromatin structures. These novel applications will likely elevate CRISPR tools into yet another level of sophistication and diversity, leading to many more exciting cell biological discoveries.
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