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Updated: Feb 22, 2026

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
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Synthesis and Analysis of Bacterial Folate Metabolism Intermediates and Antifolates
Surendra Dawadi1, Shannon L Kordus2, Anthony D Baughn2
1Department of Medicinal Chemistry, University of Minnesota , Minneapolis, Minnesota 55455, United States.
Abstract:
The mechanism of action of para-aminosalicylic acid (PAS), a drug used to treat drug-resistant tuberculosis (TB), has been confirmed through the first synthesis and biochemical characterization of its active metabolite 7. The synthesis features the coupling of N2-acetyl-6-formylpterin obtained from the degradation of folic acid and appropriately functionalized arylamines to form Schiff bases. The sequential chemoselective reduction of the imine and pterin ring led to the formation of dihydrofolate analogue 7 and two other dihydropteroate species.
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