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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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Optimizing Stem Length To Improve Ligand Selectivity in a Structure-Switching Cocaine-Binding Aptamer.

Miguel A D Neves1, Aron A Shoara1, Oren Reinstein1

  • 1Department of Chemistry and Centre for Research on Biomolecular Interactions, York University , Toronto, Ontario M3J 1P3, Canada.

ACS Sensors
|September 21, 2017
PubMed
Summary

Aptamer stem length controls structure-switching for biosensors. Modifying stem 1 length in cocaine-binding aptamers tunes selectivity, enabling quinine binding over cocaine by altering aptamer stability.

Keywords:
aptamer designbiosensorcocaine-binding aptamercoupled folding and bindingligand binding thermodynamics

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