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Mapping the interaction sites of Mucin 1 and DNA aptamer by atomic force microscopy
Nan Wang1, Miaomiao Zhang, Xuejuan Chen
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. jltang@ciac.ac.cn.
The Analyst
|September 21, 2017
Abstract:
Mucin 1 (MUC1) is an attractive tumor marker for cancer diagnosis. An advanced atomic force microscopy (AFM) mode, peak-force tapping AFM with an aptamer functionalized tip, was introduced to map the specific interaction sites of an aptamer and MUC1. Single molecular force spectroscopy (SMFS) was used to investigate dynamic parameters of the aptamer-MUC1.

