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Natural reduced water suppressed anxiety and protected the heightened oxidative stress in rats
Koji Masuda1, Yoshihiro Tanaka1, Masayuki Kanehisa1
1Department of Neuropsychiatry, Oita University Faculty of Medicine, Hasama-Machi, Oita, Japan.
Background:
In Japan, the effects of reduced water, such as hydrogen-rich electrolyzed reduced water and natural reduced water, like Hita Tenryosui water®, have been examined. The purpose of the present study was to identify the role of natural reduced water in anxiety and blood biochemical analysis.
Materials And Methods:
Natural reduced water and distilled water were administered to rats for 180 consecutive days, and their effect on anxiety-like behavior and depression was examined by using elevated plus maze, light/dark, forced swimming, and conditioned fear tests. Before and after administration of natural reduced or distilled water, we performed blood and urine analyses.
Results:
Natural reduced water exhibited anxiolytic-like effects in the conditioned fear and elevated plus maze tests. The mean levels of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the natural reduced water were significantly lower than the distilled water group. Natural reduced water group also showed decrease in blood-urea nitrogen levels compared with the distilled water group.
Conclusion:
These results indicate that natural reduced water may decrease anxiety-related behaviors and prevent heightened oxidative stress.
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